1R8M

SEC7 DOMAIN OF THE ARF EXCHANGE FACTOR ARNO WITH BREFELDIN A-SENSITIZING MUTATIONS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	33.429, 95.775, 33.665
Unit cell angles	90.00, 101.67, 90.00

Refinement procedure

Resolution	33.000 * - 1.700
R-factor	0.19414
Rwork	0.192
R-free	0.23200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pbv
RMSD bond length	0.025
RMSD bond angle	1.942
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	1.750
High resolution limit [Å]	1.700	1.700
Rmerge	0.066	0.122
Number of reflections	21082
<I/σ(I)>	18	5
Completeness [%]		65.5
Redundancy	4.5	1.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	16 *	20% PEG 3350, 190mM Sodium Formate, 100mM Hepes, 18mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	8	16 *	20% PEG 3350, 190mM Sodium Formate, 100mM Hepes, 18mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	8	16 *	20% PEG 3350, 190mM Sodium Formate, 100mM Hepes, 18mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.1 (mM)
2	1	reservoir	Tris	20 (mM)	pH8
3	1	reservoir		20 (mM)
4	1	reservoir	beta-mercaptoethanol	2.5 (mM)