1R4N
APPBP1-UBA3-NEDD8, an E1-ubiquitin-like protein complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-07-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.000, 197.900, 211.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.269 |
| Rwork | 0.251 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r4m |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.450 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.087 | 0.361 |
| Total number of observations | 2710454 * | |
| Number of reflections | 66111 * | |
| <I/σ(I)> | 12.6 | 1.9 |
| Completeness [%] | 97.9 | 94.4 |
| Redundancy | 41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 18 * | peg10k, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG10000 | 10.5-10.7 (%) | |
| 10 | 1 | drop | dithiothreitol | 5 (mM) | pH7.6 |
| 11 | 1 | drop | protein | 15-30 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 0.1 (M) | |
| 3 | 1 | reservoir | 0.4 (M) | ||
| 4 | 1 | reservoir | PEG400 | 10 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) | pH8.0-8.5 |
| 6 | 1 | drop | Tris | 50 (mM) | |
| 7 | 1 | drop | 150 (mM) | ||
| 8 | 1 | drop | 5 (mM) | ||
| 9 | 1 | drop | ATP | 5 (mM) |
