1Q3X
Crystal structure of the catalytic region of human MASP-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-12-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.950, 41.521, 102.994 |
| Unit cell angles | 96.44, 91.77, 119.52 |
Refinement procedure
| Resolution | 50.640 - 2.230 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1elv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.904 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.640 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.083 | 0.255 |
| Number of reflections | 27022 | |
| <I/σ(I)> | 7.6 | 2.9 |
| Completeness [%] | 94.8 | 74.3 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | PEG 6000, sodium chloride, glycerol, Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50 |
