1PX5

Crystal structure of the 2'-specific and double-stranded RNA-activated interferon-induced antiviral protein 2'-5'-oligoadenylate synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-23
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	46.524, 132.601, 57.305
Unit cell angles	90.00, 99.19, 90.00

Refinement procedure

Resolution	20.000 * - 1.740
R-factor	0.187
Rwork	0.187
R-free	0.22420 *
Structure solution method	SIRAS
RMSD bond length	0.004
RMSD bond angle	1.180 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.800
High resolution limit [Å]	1.740	1.740
Rmerge	0.042	0.219 *
Number of reflections	69379
<I/σ(I)>	32.4	8.1
Completeness [%]	99.4 *	96.7
Redundancy	6.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	20 *	PEG 2000mme, sodium cacodylate, ammonium sulfate, sodium chloride, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	PEG2000 MME	30 (%)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)	pH6.