1P9M

Crystal structure of the hexameric human IL-6/IL-6 alpha receptor/gp130 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	200
Detector technology	CCD
Collection date	2002-09-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	279.800, 279.800, 96.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 3.650
R-factor	0.282
Rwork	0.282
R-free	0.33400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1alu
RMSD bond length	0.005
RMSD bond angle	22.400 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.840
High resolution limit [Å]	3.650	3.650
Rmerge	0.065	0.551
Total number of observations	120957 *
Number of reflections	16144 *
<I/σ(I)>	13	1.85
Completeness [%]	98.3	98.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	Sodium formate, Sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	sodium formate	2.0 (M)	pH4.5
3	1	reservoir	sodium acetate	0.1 (M)	pH4.5