1P6O
The crystal structure of yeast cytosine deaminase bound to 4(R)-hydroxyl-3,4-dihydropyrimidine at 1.14 angstroms.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.880, 70.417, 71.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.140 |
| R-factor | 0.1097 |
| Rwork | 0.110 |
| R-free | 0.15210 |
| Structure solution method | SAD |
| Starting model (for MR) | 1ox7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.180 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.048 | 0.283 |
| Number of reflections | 92236 | |
| <I/σ(I)> | 20.84 | 3.81 |
| Completeness [%] | 92.7 | 61.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microseeding | 6.5 | 277 | PEG 8000, calcium acetate, sodium cacodylate, pH 6.5, microseeding, temperature 277K |
