1P1W

Crystal structure of the GluR2 ligand-binding core (S1S2J) with the L483Y and L650T mutations and in complex with AMPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	CCD
Collection date	2001-06-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.973
Spacegroup name	P 21 21 2
Unit cell lengths	98.883, 121.471, 47.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 1.800
R-factor	0.234
Rwork	0.234
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.220 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.053	0.283
Number of reflections	52581 *
<I/σ(I)>	16.5
Completeness [%]	96.1	90.7
Redundancy	3.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	4 *	PEG4000, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	AMPA	20 (mM)
2	1	reservoir	PEG4000	14-18 (%)
3	1	reservoir	ammonium sulfate	0.2-0.4 (M)