1NVP

HUMAN TFIIA/TBP/DNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-03-21
Detector	MARRESEARCH
Wavelength(s)	0.916, 0.909
Spacegroup name	P 21 21 21
Unit cell lengths	59.624, 90.884, 125.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 * - 2.100
R-factor	0.229
Rwork	0.229
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	25.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.065	0.362
Total number of observations	224980 *
Number of reflections	40386
<I/σ(I)>	25.6	4.1
Completeness [%]	99.8	99.8
Redundancy	5.6	3.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	22 *	PEG6000, lithium nitrate, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7 *	22 *	PEG6000, lithium nitrate, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG6000
2	1	1	lithium nitrate
3	1	1	CaCl2
4	1	2	CaCl2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
10	1	reservoir	dithiothreitol	1 (mM)
11	1	reservoir	PEG6000	12 (%(w/v))
2	1	drop	Bis-Tris	2 (mM)	pH7.0
3	1	drop		20 (mM)
4	1	drop	EDTA	0.1 (mM)
5	1	drop	dithiothreitol	0.5 (mM)
6	1	drop		0.01 (%(w/v))
7	1	reservoir	bis-Tris	50 (mM)	pH6.5
8	1	reservoir		100 (mM)
9	1	reservoir		10 (mM)