1N7S
High Resolution Structure of a Truncated Neuronal SNARE Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.767, 51.750, 132.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.130 - 1.450 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 14.300 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.140 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.690 | 0.439 |
| Number of reflections | 48574 | 4310 * |
| <I/σ(I)> | 20.4 | 3.5 |
| Completeness [%] | 97.8 | 88.4 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 4 and 20 * | MPD, CaCl2, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 4.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | HEPES | 10 (mM) | pH7.8 |
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | MPD | 15-20 (%) | |
| 6 | 1 | reservoir | 75-125 (mM) | ||
| 7 | 1 | reservoir | MES | 50 (mM) | pH5.0-6.0 |
