1MYW

CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-28
Detector	MARRESEARCH
Spacegroup name	P 31 1 2
Unit cell lengths	82.704, 82.704, 72.555
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.880 - 2.200
R-factor	0.218
Rwork	0.218
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yfp
RMSD bond length	0.001 *
RMSD bond angle	1.376 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.880	2.340
High resolution limit [Å]	2.200	2.280 *
Rmerge	0.076	0.317
Number of reflections	121451 *
<I/σ(I)>	9.2
Completeness [%]	97.7	94.5
Redundancy	8.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9 *	298	Tris, ammonium sulfate, PEG400, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	33 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.9
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	0.5 (mM)
5	1	reservoir	ammonium sulfate	2.1 (M)
6	1	reservoir	Tris-HCl	100 (mM)	pH8.4
7	1	reservoir	PEG400	4 (%(v/v))