1M6D
Crystal structure of human cathepsin F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-01-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 68.250, 103.260, 58.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.198 |
Rwork | 0.195 |
R-free | 0.22000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f2a |
RMSD bond length | 0.005 |
RMSD bond angle | 1.340 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.086 * | 0.431 * |
Total number of observations | 591413 * | |
Number of reflections | 43466 | |
<I/σ(I)> | 32 | 3.5 |
Completeness [%] | 93.7 | 64.5 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 * | 290 | magnesium acetate, sodium cacodylate, pEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6. |
3 | 1 | reservoir | PEG8000 | 25 (%(w/v)) | |
4 | 1 | drop | cathepsin | 3 (mg/ml) | |
5 | 1 | drop | sodium acetate | 50 (mM) | pH4.0 |
6 | 1 | drop | EDTA | 1 (mM) | |
7 | 1 | drop | 150 (mM) |