1M5O
Transition State Stabilization by a Catalytic RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2002-03-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 259.380, 44.220, 102.500 |
Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1HP6 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 57194 | |
<I/σ(I)> | 22.9 | 1.9 |
Completeness [%] | 99.5 | 99 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 300 | MPD, calcium Chloride, ammonium chloride, meta-ammonium vanadate, pH 5.0, VAPOR DIFFUSION, SITTING DROP at 300K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | CaCl2 | ||
2 | 1 | 1 | NH4Cl | ||
3 | 1 | 1 | meta-ammonium vanadate | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | CaCl2 | ||
6 | 1 | 2 | NH4Cl | ||
7 | 1 | 2 | MPD |