1M43
Crystal structure of PMII in complex with pepstatin a to 2.4 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 142.235, 142.235, 97.892 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.400 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sme |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.097 | |
| Number of reflections | 35751 | |
| <I/σ(I)> | 17.43 | 4.4 |
| Completeness [%] | 79.5 | 62.2 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
