1M2N

Sir2 homologues (D102G/F159A/R170A) mutant-2'-O-acetyl ADP ribose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 31 2 1
Unit cell lengths	84.720, 84.720, 193.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 * - 2.600
R-factor	0.228
Rwork	0.228
R-free	0.30600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008 *
RMSD bond angle	1.500
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.053 *
Total number of observations	81444 *
Number of reflections	19629 *
Completeness [%]	77.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	18 *	PEG 8000, HEPES, pH 7.0, EVAPORATION, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mM)
2	1	reservoir	sodium citrate	100 (mM)	pH6.0
3	1	reservoir	PEG4000	14 (%)
4	1	reservoir	isopropanol	20 (%)