1LWG
Multiple Methionine Substitutions are Tolerated in T4 Lysozyme and have Coupled Effects on Folding and Stability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.77487 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.425, 60.425, 90.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.800 - 1.700 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.844 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 | 0.284 |
| Number of reflections | 21345 | |
| <I/σ(I)> | 7.1 | 2.7 |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
