1LQB

Crystal structure of a hydroxylated HIF-1 alpha peptide bound to the pVHL/elongin-C/elongin-B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	58.940, 58.940, 243.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.000
R-factor	0.225
Rwork	0.225
R-free	0.27800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1VCB.pdb
RMSD bond length	0.006
RMSD bond angle	23.800 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.060 *	0.490 *
Total number of observations	448657 *
Number of reflections	24245
<I/σ(I)>	20	1
Completeness [%]	76.0	30
Redundancy	18.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	22 *	HEPES, PEG2000 monomethylester, ammonium sulfate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	HEPES	100 (mM)	pH7.4
3	1	reservoir	PEG2000MME	40 (%)
4	1	reservoir	ammonium sulfate	200 (mM)