1LOA

THREE-DIMENSIONAL STRUCTURES OF COMPLEXES OF LATHYRUS OCHRUS ISOLECTIN I WITH GLUCOSE AND MANNOSE: FINE SPECIFICITY OF THE MONOSACCHARIDE-BINDING SITE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	56.300, 139.800, 62.700
Unit cell angles	90.00, 91.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.179
Rwork	0.179
RMSD bond length	0.003 *
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.082 *
Total number of observations	77034 *
Number of reflections	33640 *
Completeness [%]	67.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	20 *	reservoirs contain the same ingredients as drops, but twice as consentrated. taken from Bourne, Y. et al. (1988). J. Mol. Biol., 202, 685-687. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	MPD	40 (%(v/v))
3	1	drop		0.05 (M)
4	1	drop	HEPES	0.02 (M)