1KO9

Native Structure of the Human 8-oxoguanine DNA Glycosylase hOGG1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-15
Detector	MARRESEARCH
Wavelength(s)	0.931
Spacegroup name	P 65
Unit cell lengths	105.513, 105.513, 47.597
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.070 - 2.150
R-factor	0.215 *
Rwork	0.206
R-free	0.25800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ebm
RMSD bond length	0.006
RMSD bond angle	21.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.042 *	0.250 *
Number of reflections	17891
<I/σ(I)>	13.4	3.5
Completeness [%]	99.9	100 *
Redundancy	3.5 *	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	4.6	277	PEG 4000, Ammonium Sulphate, Sodium citrate, pH 4.6, Microbatch, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	27-30 (%(w/v))
2	1	1	ammonium sulfate	0.2 (M)
3	1	1	sodium citrate	0.1 (M)	pH4.6
4	1	1	protein	10 (mg/ml)