1KCQ

Human Gelsolin Domain 2 with a Cd2+ bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-04
Detector	MARRESEARCH
Wavelength(s)	1.200
Spacegroup name	C 1 2 1
Unit cell lengths	96.968, 26.525, 50.294
Unit cell angles	90.00, 121.19, 90.00

Refinement procedure

Resolution	22.250 - 1.650
R-factor	0.18
Rwork	0.177
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Residues 151-266 from PDB ENTRY 1D0N
RMSD bond length	0.007
RMSD bond angle	1.900
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000 *	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.075	0.174
Total number of observations	44277 *
Number of reflections	13093 *
<I/σ(I)>	5.2
Completeness [%]	99.0	94.6
Redundancy	3.3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	290	PEG 400, cadmium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		50 (mM)
2	1	reservoir	sodium acetate	100 (mM)	pH4.6
3	1	reservoir	PEG400	20 (%(v/v))