1K6Y
Crystal Structure of a Two-Domain Fragment of HIV-1 Integrase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2001-03-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.2827 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.710, 102.710, 280.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.340 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.084 * | 0.356 * |
Number of reflections | 53869 * | |
Completeness [%] | 90.1 * | 84.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.2 * | 277 | 0.7 M NaH2PO4/1.0 K2HPO4, 0.1M Na acetate, pH 6.0, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | drop | 0.5 (M) | ||
3 | 1 | drop | HEPES | 20 (mM) | pH7.2 |
4 | 1 | drop | 0.050 (mM) | ||
5 | 1 | drop | dithiothreitol | 5 (mM) | |
6 | 1 | reservoir | 0.7 (M) | ||
7 | 1 | reservoir | 1.0 (M) | ||
8 | 1 | reservoir | acetate | 0.1 (M) | pH4.6 |