1K6O
Crystal Structure of a Ternary SAP-1/SRF/c-fos SRE DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-11-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0722 |
| Spacegroup name | P 63 |
| Unit cell lengths | 161.784, 161.784, 41.224 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 * |
| R-factor | 0.27 |
| Rwork | 0.220 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.175 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 3.200 * | 3.190 |
| Rmerge | 0.131 * | |
| Number of reflections | 10302 | |
| Completeness [%] | 97.1 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000, pH 6.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | SAP-1 | 0.1 (mM) | |
| 2 | 1 | drop | SRF dimer | 0.1 (mM) | |
| 3 | 1 | drop | SRE DNA duplex | 0.13 (mM) | |
| 4 | 1 | drop | MES | 25 (mM) | pH6.0 |
| 5 | 1 | drop | PEG2000 | 8 (%) | |
| 6 | 1 | drop | 5 (mM) |
