1JL0
Structure of a Human S-Adenosylmethionine Decarboxylase Self-processing Ester Intermediate and Mechanism of Putrescine Stimulation of Processing as Revealed by the H243A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 1999-06-17 |
| Detector | SBC-1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.760, 55.990, 90.210 |
| Unit cell angles | 90.00, 109.72, 90.00 |
Refinement procedure
| Resolution | 19.910 - 1.500 |
| R-factor | 0.215 * |
| Rwork | 0.215 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jen |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 0.275 |
| Number of reflections | 111488 | |
| <I/σ(I)> | 17.9 | 4.6 |
| Completeness [%] | 98.8 | 99.5 |
| Redundancy | 3.96 | 3.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | PEG 8000, dithiothreitol, tris(hydroxymethyl)aminomethane, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 15 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) |
