1J5O

CRYSTAL STRUCTURE OF MET184ILE MUTANT OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH DOUBLE STRANDED DNA TEMPLATE-PRIMER

Replaces:  1C9R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-09-10
Detector	FUJI
Spacegroup name	P 32 1 2
Unit cell lengths	169.180, 169.180, 221.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.500
R-factor	0.27
Rwork	0.262
R-free	0.33800
RMSD bond length	0.012
RMSD bond angle	1.680
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.118	0.400
Number of reflections	42062
<I/σ(I)>	11.2
Completeness [%]	91.5 *	83
Redundancy	5.91	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	4 *	30 % SATURATED AMMONIUM SULFATE, 100 MM CACODYLATE PH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.6	4 *	30 % SATURATED AMMONIUM SULFATE, 100 MM CACODYLATE PH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	DNA	0.2 (mM)
3	1	reservoir	cacodylate	100 (mM)	pH5.6
4	1	reservoir	ammonium sulfate	29-31 (%sat)