1ISS

Crystal Structure of Metabotropic Glutamate Receptor Subtype 1 Complexed with an antagonist

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-12-01
Detector	RIGAKU RAXIS V
Wavelength(s)	0.836
Spacegroup name	P 41 21 2
Unit cell lengths	112.142, 112.142, 289.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.300
R-factor	0.257 *
Rwork	0.257
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CHAIN A of PDB ENTRY 1EWK
RMSD bond length	0.009 *
RMSD bond angle	1.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.370
High resolution limit [Å]	3.300	3.300
Rmerge	0.086	0.493
Number of reflections	27580
<I/σ(I)>		1.6
Completeness [%]	97.8	99.3
Redundancy	4.3	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Kunishima, N., (2000) Nature, 407, 971. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.2 (M)	pH8.5
2	1	reservoir	ammonium sulfate	1.9 (M)
3	1	reservoir	S-MCPG	10 (mM)