1HRN

HIGH RESOLUTION CRYSTAL STRUCTURES OF RECOMBINANT HUMAN RENIN IN COMPLEX WITH POLYHYDROXYMONOAMIDE INHIBITORS

Experimental procedure

Spacegroup name	P 21 3
Unit cell lengths	141.100, 141.100, 141.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.215
Rwork	0.215
RMSD bond length	0.019
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.066 *
Total number of observations	489730 *
Number of reflections	77612 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *		enzyme solution resulted in a 4:1 molar excess *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	inhibitor	20 (mg/ml)
3	1	drop	DMSO	4 (%)
4	1	reservoir	sodium citrate	50 (mM)	precipitant
5	1	reservoir		0.6 (M)	precipitant
6	1	reservoir	PEG4600	20 (%(w/v))
7	1	reservoir		0.02 (%)