1HR9
Yeast Mitochondrial Processing Peptidase beta-E73Q Mutant Complexed with Malate Dehydrogenase Signal Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2000-06-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9430 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 134.370, 178.520, 201.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.550 - 3.010 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25600 |
| Starting model (for MR) | PDB ID 1HR6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.094 | 0.481 |
| Total number of observations | 418644 * | |
| Number of reflections | 90666 | |
| <I/σ(I)> | 13.6 | 2.3 |
| Completeness [%] | 93.5 | 95.9 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 21 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-14 (mg/ml) | |
| 10 | 1 | reservoir | n-dodecylmaltoside | 0.2 (mM) | |
| 11 | 1 | reservoir | sodium azide | 2 (mM) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | glycerol | 30 (%(v/v)) | |
| 4 | 1 | drop | n-dodecylmaltoside | 0.2 (mM) | |
| 5 | 1 | reservoir | PEG10000 | 5-9 (%(w/v)) | |
| 6 | 1 | reservoir | ethylene glycol | 35 (%(v/v)) | |
| 7 | 1 | reservoir | MPD | 6 (%(v/v)) | |
| 8 | 1 | reservoir | sodium citrate | 25 (mM) | |
| 9 | 1 | reservoir | benzamidine | 2 (%(w/v)) |
