1HQV

STRUCTURE OF APOPTOSIS-LINKED PROTEIN ALG-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-08
Detector	ADSC QUANTUM 4
Wavelength(s)	1.04453
Spacegroup name	P 41 2 2
Unit cell lengths	72.060, 72.060, 91.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
Rwork	0.250
R-free	0.29500
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.220 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.061	0.213
Total number of observations	69890 *
Number of reflections	10911
<I/σ(I)>	23.7
Completeness [%]	96.8	96
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 8000, Calcium Acetate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	sodium cacodylate	0.1 (M)
3	1	reservoir		100 (mM)
4	1	reservoir	PEG8000	12-15 (%)