1HF0

Crystal structure of the DNA-binding domain of Oct-1 bound to DNA as a dimer

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-15
Detector	MARRESEARCH
Wavelength(s)	0.8856, 0.9185, 0.9190, 0.8424
Spacegroup name	P 62 2 2
Unit cell lengths	131.250, 131.250, 116.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.2391
Rwork	0.239
R-free	0.29410
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.058	0.389
Number of reflections	16742
<I/σ(I)>	23	3.5
Completeness [%]	99.5	100
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.3		pH 5.30