1HE8
Ras G12V - PI 3-kinase gamma complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.635, 113.635, 183.881 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.030 - 3.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1E8Y AND 5P21 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.102 | 0.301 |
| Total number of observations | 232825 * | |
| Number of reflections | 25822 | |
| <I/σ(I)> | 20 | 1.7 |
| Completeness [%] | 91.6 | 50.5 |
| Redundancy | 9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.3 | 50 MM KH2PO4 PH 6.3, 4.3% PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | H-Ras | 0.170 (mM) | |
| 2 | 1 | drop | PI3K | 0.029 (mM) | |
| 3 | 1 | reservoir | potassium phosphate | 50 (mM) | pH6.3 |
| 4 | 1 | reservoir | PEG8000 | 4.3 (%) |
