1H2T

Structure of the human nuclear cap-binding-complex (CBC) in complex with a cap analogue m7GpppG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-05
Detector	ADSC QUANTUM 315r
Spacegroup name	P 31 2 1
Unit cell lengths	112.780, 112.780, 158.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.760 - 2.100
R-factor	0.232
Rwork	0.232
R-free	0.26600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h6k
RMSD bond length	0.006
RMSD bond angle	20.400 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.079	0.398
Number of reflections	60513 *
<I/σ(I)>	5.3	1.8
Completeness [%]	99.8	99.8
Redundancy	7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	277 *	Mazza, C., (2002) Acta Crystallogr.,Sect.D, 58, 2194. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	10 (%)
3	1	reservoir	MES	100 (mM)	pH6.