1FRB

FR-1 PROTEIN/NADPH/ZOPOLRESTAT COMPLEX

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1995-05-19
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	59.450, 65.380, 90.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.158
Rwork	0.158
RMSD bond length	0.008
Data reduction software	SDMS (DETECTOR SYSTEM (NIELSEN))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.046
Total number of observations	213331 *
Number of reflections	43337
Completeness [%]	94.3	90 *
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ALR2	7 (mg/ml)
2	1	drop	zopolrestat	0.2 (mM)
3	1	drop	2-mercaptoethanol	3.5 (mM)
4	1	drop	citrate	25 (mM)
5	1	reservoir	PEG6000	17 (%)
6	1	reservoir	2-mercaptoethanol	7 (mM)
7	1	reservoir	citrate	50 (mM)