1FB1
CRYSTAL STRUCTURE OF HUMAN GTP CYCLOHYDROLASE I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 115.110, 115.110, 387.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.970 - 3.100 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.29300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.970 | 3.290 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.165 | |
| Total number of observations | 276838 * | |
| Number of reflections | 25288 | |
| Completeness [%] | 84.5 | 41.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | ammonium sulphate, isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.6 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 100 (mM) | |
| 3 | 1 | drop | 1,4-dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | 0.02 (%) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 6 | 1 | reservoir | isopropanol | 3.2 (%) | |
| 7 | 1 | reservoir | dithiothreitol | 2 (mM) |
