1EER

CRYSTAL STRUCTURE OF HUMAN ERYTHROPOIETIN COMPLEXED TO ITS RECEPTOR AT 1.9 ANGSTROMS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	58.400, 79.300, 136.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.242
Rwork	0.242
R-free	0.31800
Structure solution method	MIRAS
RMSD bond length	0.006
RMSD bond angle	27.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.044 *	0.377 *
Total number of observations	251681 *
Number of reflections	49480
<I/σ(I)>	19.1	2.5
Completeness [%]	97.0	82.9
Redundancy	5.1	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	20 *	pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG1500	32 (%)
2	1	reservoir	ammonium sulfate	0.28 (M)
3	1	reservoir	MES	0.1 (M)	pH6.5