1E0O
CRYSTAL STRUCTURE OF A TERNARY FGF1-FGFR2-HEPARIN COMPLEX
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 |
| Unit cell lengths | 195.900, 195.900, 68.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.200 - 2.800 |
| R-factor | 0.242 * |
| Rwork | 0.274 |
| R-free | 0.28000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.520 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.200 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.084 * | 0.474 * |
| Total number of observations | 127885 * | |
| Number of reflections | 36342 | |
| <I/σ(I)> | 13.7 | 1.7 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 18 * | CRYSTALS WERE GROWN FROM: 1.0M LI2SO4, 0.1M TRISCL PH=8.5, 10MM NISO4, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.1 (mM) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | HEPES | 10 (mM) | |
| 4 | 1 | drop | 25 (mM) | ||
| 5 | 1 | reservoir | 1.0 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 7 | 1 | reservoir | 10 (mM) |
