1DHF

CRYSTAL STRUCTURES OF RECOMBINANT HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 5-DEAZOFOLATE

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	36.100, 38.700, 76.900
Unit cell angles	94.30, 91.70, 111.00

Refinement procedure

Resolution	? - 2.300
R-factor	0.176
RMSD bond length	0.020
RMSD bond angle	0.033
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.056 *
Number of reflections	16023 *
Completeness [%]	91.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.9 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	0.025 (M)
2	1	drop	dithioerythritol	0.5 (mM)
3	1	drop	sodium folate		3-fold molar excess of substrate to enzyme
4	1	drop	sodium acetate	0.30 (M)
5	1	drop	PEG4000	35 (%(w/v))
6	1	reservoir	sodium acetate	0.17 (M)
7	1	reservoir	PEG4000	20 (%(w/v))
8	1	reservoir	ethanol	7 (%(v/v))