1D8T
CRYSTAL STRUCTURE OF ELONGATION FACTOR, TU (EF-TU-MGGDP) COMPLEXED WITH GE2270A, A THIAZOLYL PEPTIDE ANTIBIOTIC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-18 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.470, 45.170, 144.000 |
| Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
| Resolution | 24.440 - 2.350 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.24600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA) |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.440 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.032 | 0.108 |
| Total number of observations | 55193 * | |
| Number of reflections | 31214 | |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 86.3 | 60.7 |
| Redundancy | 1.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 20 * | TRIS-HCL, MAGNESIUM CHLORIDE, GDP, POLYETHYLENE GLYCOL 3350, AMMONIUM ACETATE, AMMONIUM CITRATE, PH 7.6, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293.K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.4 (mg/ml) | |
| 2 | 1 | drop | PEG3350 | 20 (%(w/v)) | |
| 3 | 1 | drop | ammonium acetate | 0.2 (M) | |
| 4 | 1 | drop | ammonium citrate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG8000 | 50 (%(w/v)) |
