1D5R

Crystal Structure of the PTEN Tumor Suppressor

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	130
Detector technology	CCD
Collection date	1998-12-20
Detector	ADSC QUANTUM 4
Spacegroup name	I 4
Unit cell lengths	113.140, 113.140, 58.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.219 *
Rwork	0.224
R-free	0.27600
RMSD bond length	0.009
RMSD bond angle	1.660
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.037	0.163
Total number of observations	188143 *
Number of reflections	20302
<I/σ(I)>	42.2
Completeness [%]	95.8	76.4
Redundancy	3.5	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	NA/K TARTRATE, 5% GLYCEROL, 100MM TRIS-CL, 10MM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Na/K L(+)-tartrate	1.3 (M)
2	1	reservoir	glycerol	5-10 (%(v/v))
3	1	reservoir	Tris-Cl	100 (mM)
4	1	reservoir	dithiothreitol	10 (mM)