1CZ8

VASCULAR ENDOTHELIAL GROWTH FACTOR IN COMPLEX WITH AN AFFINITY MATURED ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01-28
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	89.120, 66.400, 138.750
Unit cell angles	90.00, 94.70, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.208
Rwork	0.208
R-free	0.26700
RMSD bond length	0.012
RMSD bond angle	29.600 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	X-PLOR
Refinement software	X-PLOR (98.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.076	0.380
Total number of observations	209257 *
Number of reflections	61742	8900 *
<I/σ(I)>	13.6
Completeness [%]	97.4	96.7
Redundancy	3.4	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6	293	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.8 (A280)
2	1	drop	Tris-HCl	30 (mM)
3	1	drop	sodium chloride	0.4 (M)
4	1	reservoir	PEG	15 (%)
5	1	reservoir	isopropanol	10 (%)
6	1	reservoir	ammonium sulfate	0.2 (M)
7	1	reservoir	MES	0.2 (M)