1CXZ

CRYSTAL STRUCTURE OF HUMAN RHOA COMPLEXED WITH THE EFFECTOR DOMAIN OF THE PROTEIN KINASE PKN/PRK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-C
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1999-01-05
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 41 21 2
Unit cell lengths	66.900, 66.900, 149.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.800 - 2.200
R-factor	0.214
Rwork	0.214
R-free	0.26800
RMSD bond length	0.006
RMSD bond angle	22.450 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.312
Total number of observations	696854 *
Number of reflections	17568
<I/σ(I)>	24
Completeness [%]	96.0	86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.5	4 *	Maesaki, R., (1999) J.Struct.Biol., 126, 166. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	2 (mg/ml)
2	1	1	Bis-Tris	50 (mM)
3	1	1	calcium acetate	15 (mM)
4	1	1	PEG300	15 (%)