1C8K
FLAVOPIRIDOL INHIBITS GLYCOGEN PHOSPHORYLASE BY BINDING AT THE INHIBITOR SITE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 287 |
Collection date | 2000-03-01 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.400, 128.400, 116.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.500 - 1.760 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.24400 |
Structure solution method | OTHER |
RMSD bond length | 0.008 |
RMSD bond angle | 25.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.053 | 0.640 |
Total number of observations | 489494 * | |
Number of reflections | 95442 | |
<I/σ(I)> | 14.6 | 2 |
Completeness [%] | 99.3 | 97.5 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | other * | 6.7 | pH 6.70 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | enzyme | 25 (mg/ml) | |
2 | 1 | 1 | flavopiridol | 1 (mM) | |
3 | 1 | 1 | dithiothreitol | 3 (mM) | |
4 | 1 | 1 | Bes | 10 (mM) | |
5 | 1 | 1 | EDTA | 0.1 (mM) | |
6 | 1 | 1 | sodium azide | 0.02 (%) | |
7 | 1 | 1 | spermine | 1 (mM) | or 10 mM magnesium acetate |
8 | 1 | 1 | glucose | 50 (mM) |