1BKC
CATALYTIC DOMAIN OF TNF-ALPHA CONVERTING ENZYME (TACE)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.380, 126.270, 81.270 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.000 |
| Rwork | 0.180 |
| R-free | 0.27000 |
| Structure solution method | MULTIWAVELENGTH ANOMALOUS DISPERSION |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.031 | |
| Number of reflections | 77653 | |
| Completeness [%] | 96.9 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.4 | pH 5.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | isopropanol | 20 (%) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | citrate | 0.1 (M) |
