1BI7
MECHANISM OF G1 CYCLIN DEPENDENT KINASE INHIBITION FROM THE STRUCTURE OF THE CDK6-P16INK4A TUMOR SUPPRESSOR COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1997-01 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 123.100, 123.100, 112.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.400 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.33000 |
| Structure solution method | MIR, MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.940 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.068 * | 0.330 * |
| Total number of observations | 68633 * | |
| Number of reflections | 12443 | |
| <I/σ(I)> | 17.7 | 6.9 |
| Completeness [%] | 97.7 | 96.2 |
| Redundancy | 5.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | isopropanol | 11 (%) | |
| 2 | 1 | reservoir | HEPES-Na | 40 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 5 (mM) |
