1AXI

STRUCTURAL PLASTICITY AT THE HGH:HGHBP INTERFACE

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1996-05-05
Detector	ADSC
Spacegroup name	P 43 21 2
Unit cell lengths	65.600, 65.600, 231.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.100
Rwork	0.190
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HGH(G120R):HGHBP STRUCTURE (IN PREPARATION)
RMSD bond length	0.014
RMSD bond angle	0.035
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.057	0.125
Total number of observations	133933 *
Number of reflections	28276
<I/σ(I)>		7.4
Completeness [%]	91.8	51.3
Redundancy	4.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)
2	1	1	Bis-Tris	50 (mM)
3	1	1	ammonium sulfate	17-20 (%sat)