12FH

Structure of eIF2B bound to a activator

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	131.470, 230.810, 89.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.980 - 3.500
R-factor	0.2177
Rwork	0.215
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.929
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.000	3.300
High resolution limit [Å]	3.100	3.100
Rmerge		1.180
Number of reflections	38403	1920
<I/σ(I)>	10.9
Completeness [%]	94.1
Redundancy	6.6
CC(1/2)	0.998	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.9 M (NH4)2SO4, 0.45 % PEG8K, and 0.09 M HEPES pH 7.0.