11UE
Structure of PDGFRb Kinase Domain Bound to JNJ-PDGFRBi-1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-18 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.400, 97.400, 101.687 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.920 - 2.320 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.567 |
| Data reduction software | XDS (20220220) |
| Data scaling software | autoPROC |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.351 | 2.356 |
| High resolution limit [Å] | 2.316 | 2.316 |
| Rmerge | 0.136 | 1.098 |
| Rmeas | 0.144 | 1.156 |
| Rpim | 0.046 | 0.360 |
| Number of reflections | 24715 | 1197 |
| <I/σ(I)> | 9.283 | 2.088 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 10.21 |
| CC(1/2) | 0.994 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES PH 6.5, 11% PEG 550 MME |
