+Open data
-Basic information
Entry | Database: PDB / ID: 2mxe | ||||||
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Title | Solution structure of the C-terminal domain of MvaT | ||||||
Components | MvaT | ||||||
Keywords | TRANSCRIPTION REGULATOR | ||||||
Function / homology | MvaT, DNA-binding domain / negative regulation of single-species biofilm formation on inanimate substrate / negative regulation of secondary metabolite biosynthetic process / DNA-binding transcription activator activity / protein-DNA complex / transcription cis-regulatory region binding / positive regulation of DNA-templated transcription / MvaT Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa PAO1 (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Ding, P. / Xia, B. | ||||||
Citation | Journal: Plos Pathog. / Year: 2015 Title: A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT. Authors: Ding, P. / McFarland, K.A. / Jin, S. / Tong, G. / Duan, B. / Yang, A. / Hughes, T.R. / Liu, J. / Dove, S.L. / Navarre, W.W. / Xia, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mxe.cif.gz | 294.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mxe.ent.gz | 255.3 KB | Display | PDB format |
PDBx/mmJSON format | 2mxe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mx/2mxe ftp://data.pdbj.org/pub/pdb/validation_reports/mx/2mxe | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6460.413 Da / Num. of mol.: 1 / Fragment: C-terminal domain (UNP residues 77-124) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa PAO1 (bacteria) / Strain: PAO1 / Gene: mvaT, PA4315 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9HW86 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM [U-100% 13C; U-100% 15N] MvaT, 50 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.1 / pH: 6 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: AMBER Developer: Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR constraints | NOE constraints total: 1820 / NOE intraresidue total count: 557 / NOE long range total count: 242 / NOE medium range total count: 177 / NOE sequential total count: 299 / Protein chi angle constraints total count: 7 / Protein other angle constraints total count: 8 / Protein phi angle constraints total count: 32 / Protein psi angle constraints total count: 32 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |