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Yorodumi- PDB-5lxj: Solution NMR structure of the X domain of Peste des Petits Rumina... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5lxj | ||||||
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Title | Solution NMR structure of the X domain of Peste des Petits Ruminants phosphoprotein | ||||||
Components | Phosphoprotein | ||||||
Keywords | VIRAL PROTEIN / XD domain Nucleocapsid binding domain / STRUCTURE FROM CYANA 3.97 | ||||||
Function / homology | Function and homology information viral genome replication / viral nucleocapsid / RNA-dependent RNA polymerase activity / DNA-templated transcription / RNA binding Similarity search - Function | ||||||
Biological species | Peste-des-petits-ruminants virus | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Pereira, N. / Piuzzi, M. / Bontems, F. / Eleouet, J.-F. / Sizun, C. | ||||||
Citation | Journal: To Be Published Title: Solution structure of the X domain of Peste des Petits Ruminants Virus phosphoprotein and interaction with the nucleoprotein Authors: Pereira, N. / Basbous, N. / Piuzzi, M. / Bontems, F. / Eleouet, J.-F. / Sizun, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5lxj.cif.gz | 385 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5lxj.ent.gz | 327.6 KB | Display | PDB format |
PDBx/mmJSON format | 5lxj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lx/5lxj ftp://data.pdbj.org/pub/pdb/validation_reports/lx/5lxj | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5964.977 Da / Num. of mol.: 1 / Fragment: X domain, UNP residues 459-509 Source method: isolated from a genetically manipulated source Details: This peptide contains residues S459-P509. The two N-terminal GS residues are left from an N-terminal GST-tag cleaved with thrombin. Source: (gene. exp.) Peste-des-petits-ruminants virus / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q91QS4 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 300 mM / Ionic strength err: 30 / Label: 288K / pH: 7.4 / Pressure: 1 bar / Temperature: 288.0 K / Temperature err: 0.2 |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 5 | ||||||||||||||||||||||||
NMR representative | Selection criteria: target function | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |