SASDF86: Human Galectin-10 (Tyr69Glu mutant) (Galectin-10 Tyr69Glu, Gal-10)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDF86

試料

Human Galectin-10 (Tyr69Glu mutant)

• Galectin-10 Tyr69Glu (protein), Gal-10, Homo sapiens

機能・相同性

分子機能:

regulation of activated T cell proliferation / regulation of T cell cytokine production / T cell apoptotic process / regulation of T cell anergy / carbohydrate binding / : / identical protein binding / cytosol

ドメイン・相同性:

Galectin-like / Galactoside-binding lectin / Galectin / Galectin, carbohydrate recognition domain / Galactoside-binding lectin / Galactoside-binding lectin (galectin) domain profile. / Concanavalin A-like lectin/glucanase domain superfamily

構成要素:

Galectin-10

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: Science / 年: 2019; タイトル: Protein crystallization promotes type 2 immunity and is reversible by antibody treatment.; 著者: Emma K Persson / Kenneth Verstraete / Ines Heyndrickx / Elien Gevaert / Helena Aegerter / Jean-Michel Percier / Kim Deswarte / Koen H G Verschueren / Ann Dansercoer / Delphine Gras / Pascal Chanez / Claus Bachert / Amanda Gonçalves / Hanne Van Gorp / Hans De Haard / Christophe Blanchetot / Michael Saunders / Hamida Hammad / Savvas N Savvides / Bart N Lambrecht / ; 要旨: Although spontaneous protein crystallization is a rare event in vivo, Charcot-Leyden crystals (CLCs) consisting of galectin-10 (Gal10) protein are frequently observed in eosinophilic diseases, such as asthma. We found that CLCs derived from patients showed crystal packing and Gal10 structure identical to those of Gal10 crystals grown in vitro. When administered to the airways, crystalline Gal10 stimulated innate and adaptive immunity and acted as a type 2 adjuvant. By contrast, a soluble Gal10 mutein was inert. Antibodies directed against key epitopes of the CLC crystallization interface dissolved preexisting CLCs in patient-derived mucus within hours and reversed crystal-driven inflammation, goblet-cell metaplasia, immunoglobulin E (IgE) synthesis, and bronchial hyperreactivity (BHR) in a humanized mouse model of asthma. Thus, protein crystals may promote hallmark features of asthma and are targetable by crystal-dissolving antibodies.

登録者

• Clement Blanchet
• Kenneth Verstraete

モデル

• モデル #2989: ; タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 11.2234292988744; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Human Galectin-10 (Tyr69Glu mutant) / 試料濃度: 20 mg/ml
• バッファ: 名称: 20 mM Hepes 150 NaCl / pH: 7.4

要素 #1642

名称: Gal-10 / タイプ: protein / 記述: Galectin-10 Tyr69Glu / 分子量: 16.613 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q05315
配列:

GHMSLLPVPY TEAASLSTGS TVTIKGRPLA CFLNEPYLQV DFHTEMKEES DIVFHFQVCF GRRVVMNSRE EGAWKQQVES KNMPFQDGQE FELSISVLPD KYQVMVNGQS SYTFDHRIKP EAVKMVQVWR DISLTKFNVS YLKR

実験情報

• ビーム: 設備名称: SOLEIL SWING / 地域: Saint-Aubin / 国: France / 線源: X-ray synchrotron / 波長: 0.103 Å / スペクトロメータ・検出器間距離: 1.8 mm
• 検出器: 名称: Eiger 4M / Pixsize x: 75 mm

スキャン

タイトル: Galactin 10 / 測定日: 2018年2月4日 / セル温度: 15 °C / 照射時間: 1 sec. / フレーム数: 14 / 単位: 1/A /

	Min	Max
Q	0.0066	0.6089

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.5a / ポイント数: 470 /

	Min	Max
Q	0.007348	0.4816
P(R) point	1	470
R	0	81.5

結果

カーブのタイプ: sec /

	実験値	Porod
分子量	30.2 kDa	28.7 kDa
体積	-	46 nm3

	Guinier	P(R)	Guinier error
前方散乱 I0	0.120254	-	-
慣性半径, Rg	2.147 nm	2.172 nm	0.01

	Min	Max
D	-	8.15
Guinier point	15	102