[English] 日本語
Yorodumi- SASDF86: Human Galectin-10 (Tyr69Glu mutant) (Galectin-10 Tyr69Glu, Gal-10) -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDF86 |
---|---|
Sample | Human Galectin-10 (Tyr69Glu mutant)
|
Function / homology | Function and homology information regulation of activated T cell proliferation / regulation of T cell cytokine production / T cell apoptotic process / regulation of T cell anergy / cysteine-type endopeptidase activity involved in apoptotic process / carbohydrate binding / collagen-containing extracellular matrix / identical protein binding / cytosol Similarity search - Function |
Biological species | Homo sapiens (human) |
Citation | Journal: Science / Year: 2019 Title: Protein crystallization promotes type 2 immunity and is reversible by antibody treatment. Authors: Emma K Persson / Kenneth Verstraete / Ines Heyndrickx / Elien Gevaert / Helena Aegerter / Jean-Michel Percier / Kim Deswarte / Koen H G Verschueren / Ann Dansercoer / Delphine Gras / Pascal ...Authors: Emma K Persson / Kenneth Verstraete / Ines Heyndrickx / Elien Gevaert / Helena Aegerter / Jean-Michel Percier / Kim Deswarte / Koen H G Verschueren / Ann Dansercoer / Delphine Gras / Pascal Chanez / Claus Bachert / Amanda Gonçalves / Hanne Van Gorp / Hans De Haard / Christophe Blanchetot / Michael Saunders / Hamida Hammad / Savvas N Savvides / Bart N Lambrecht / Abstract: Although spontaneous protein crystallization is a rare event in vivo, Charcot-Leyden crystals (CLCs) consisting of galectin-10 (Gal10) protein are frequently observed in eosinophilic diseases, such ...Although spontaneous protein crystallization is a rare event in vivo, Charcot-Leyden crystals (CLCs) consisting of galectin-10 (Gal10) protein are frequently observed in eosinophilic diseases, such as asthma. We found that CLCs derived from patients showed crystal packing and Gal10 structure identical to those of Gal10 crystals grown in vitro. When administered to the airways, crystalline Gal10 stimulated innate and adaptive immunity and acted as a type 2 adjuvant. By contrast, a soluble Gal10 mutein was inert. Antibodies directed against key epitopes of the CLC crystallization interface dissolved preexisting CLCs in patient-derived mucus within hours and reversed crystal-driven inflammation, goblet-cell metaplasia, immunoglobulin E (IgE) synthesis, and bronchial hyperreactivity (BHR) in a humanized mouse model of asthma. Thus, protein crystals may promote hallmark features of asthma and are targetable by crystal-dissolving antibodies. |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Models
Model #2989 | Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 11.2234292988744 Search similar-shape structures of this assembly by Omokage search (details) |
---|
-Sample
Sample | Name: Human Galectin-10 (Tyr69Glu mutant) / Specimen concentration: 20 mg/ml |
---|---|
Buffer | Name: 20 mM Hepes 150 NaCl / pH: 7.4 |
Entity #1642 | Name: Gal-10 / Type: protein / Description: Galectin-10 Tyr69Glu / Formula weight: 16.613 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: Q05315 Sequence: GHMSLLPVPY TEAASLSTGS TVTIKGRPLA CFLNEPYLQV DFHTEMKEES DIVFHFQVCF GRRVVMNSRE EGAWKQQVES KNMPFQDGQE FELSISVLPD KYQVMVNGQS SYTFDHRIKP EAVKMVQVWR DISLTKFNVS YLKR |
-Experimental information
Beam | Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.8 mm | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: Eiger 4M / Pixsize x: 75 mm | ||||||||||||||||||||||||||||||
Scan | Title: Galactin 10 / Measurement date: Feb 4, 2018 / Cell temperature: 15 °C / Exposure time: 1 sec. / Number of frames: 14 / Unit: 1/A /
| ||||||||||||||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 4.5a / Number of points: 470 /
| ||||||||||||||||||||||||||||||
Result | Type of curve: sec /
|