SASDE59: Interleukin-1 receptor type 1, IL-1RI-ECD (Interleukin-1 receptor...

Basic information

• Entry: Database: SASBDB / ID: SASDE59

Sample

Interleukin-1 receptor type 1, IL-1RI-ECD

• Interleukin-1 receptor type 1 (protein), IL-1R-1, Homo sapiens

Function / homology

Function:

positive regulation of interleukin-1-mediated signaling pathway / interleukin-1, type I, activating receptor activity / positive regulation of neutrophil extravasation / interleukin-1 receptor activity / interleukin-1 binding / smooth muscle adaptation / positive regulation of T-helper 1 cell cytokine production / ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase / positive regulation of platelet-derived growth factor receptor signaling pathway / NAD+ nucleosidase activity, cyclic ADP-ribose generating / platelet-derived growth factor receptor binding / interleukin-1-mediated signaling pathway / Interleukin-10 signaling / response to interleukin-1 / positive regulation of type II interferon production / Interleukin-1 signaling / transmembrane signaling receptor activity / protease binding / regulation of inflammatory response / Potential therapeutics for SARS / positive regulation of canonical NF-kappaB signal transduction / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / cell surface receptor signaling pathway / immune response / inflammatory response / external side of plasma membrane / cell surface / extracellular region / membrane / plasma membrane

Domain/homology:

Interleukin-1 receptor type 1 / Interleukin-1 receptor type I/II / IL-1Ra-like, immunoglobulin domain / Immunoglobulin domain / Interleukin-1 receptor family / TIR domain / Immunoglobulin domain / Toll - interleukin 1 - resistance / TIR domain profile. / Toll/interleukin-1 receptor homology (TIR) domain / Toll/interleukin-1 receptor homology (TIR) domain superfamily / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold

Component:

Interleukin-1 receptor type 1

• Biological species: Homo sapiens (human)
• Citation: Journal: Structure / Year: 2019; Title: Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.; Authors: Jiwan Ge / Soumya G Remesh / Michal Hammel / Si Pan / Andrew D Mahan / Shuying Wang / Xinquan Wang / ; Abstract: The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 cytokines. The inter-domain flexibility of IL-1 receptors and its functional roles have not been fully elucidated. In this study, we used small-angle X-ray scattering to show that ligand-binding primary receptors and co-receptors in the family all have inherent inter-domain flexibility due to the D2/D3 linker. Variants of the IL-1RAcP and IL-18Rβ co-receptors with mutated D2/D3 linkers cannot form a cytokine-receptor complex and mediate signaling. Our analysis further revealed that these mutated co-receptors exhibited a changed conformational ensemble, suggesting that loss of function is due to the alteration of receptor dynamics. Taken together, our results demonstrate that the D2/D3 linker is a critical functional determinant of IL-1 receptor and underscore the important roles of the inter-domain flexibility in cytokine/receptor binding and signaling.

Contact author

• Shu-Ying Wang (National Cheng Kung University, Taiwan)

Models

• Model #2681: ; Type: atomic / Chi-square value: 1.2737678614; Search similar-shape structures of this assembly by Omokage search (details)
• Model #2682: ; Type: atomic / Chi-square value: 1.2737678614; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Interleukin-1 receptor type 1, IL-1RI-ECD / Specimen concentration: 10 mg/ml
• Buffer: Name: 10mM HEPES, 150mM NaCl, 3% glycerol / pH: 7.2

Entity #1393

Name: IL-1R-1 / Type: protein / Description: Interleukin-1 receptor type 1 / Formula weight: 36.899 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P14778
Sequence:

LEADKCKERE EKIILVSSAN EIDVRPCPLN PNEHKGTITW YKDDSKTPVS TEQASRIHQH KEKLWFVPAK VEDSGHYYCV VRNSSYCLRI KISAKFVENE PNLCYNAQAI FKQKLPVAGD GGLVCPYMEF FKNENNELPK LQWYKDCKPL LLDNIHFSGV KDRLIVMNVA EKHRGNYTCH ASYTYLGKQY PITRVIEFIT LEENKPTRPV IVSPANETME VDLGSQIQLI CNVTGQLSDI AYWKWNGSVI DEDDPVLGED YYSVENPANK RRSTLITVLN ISEIESRFYK HPFTCFAKNT HGIDAAYIQL IYPVHHHHHH

Experimental information

• Beam: Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS); City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.5 mm
• Detector: Name: Pilatus3 X 2M / Pixsize x: 172 mm

Scan

Title: Interleukin-1 receptor type 1, IL-1RI-ECD / Measurement date: Jul 22, 2017 / Cell temperature: 20 °C / Exposure time: 3 sec. / Number of frames: 600 / Unit: 1/A /

	Min	Max
Q	0.0149	0.3293

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 404 /

	Min	Max
Q	0.02037	0.2654
P(R) point	10	413
R	0	105.4

Result

Type of curve: sec
Comments: SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.

	Experimental	Porod
MW	43.6 kDa	-
Volume	-	63 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	147.5	142.54	0.05
Radius of gyration, Rg	3.2 nm	3 nm	0.02

	Min	Max
D	-	10.54
Guinier point	1	48